Web6 sep. 2012 · Mechanical properties of cardiomyocytes were assessed using an IonOptix MyoCam system (IonOptix, Milton, MA). In brief, myocytes were placed in a chamber mounted on the stage of an inverted microscope and superfused with a buffer containing (in mmol/l) 131 NaCl, 4 KCl, 1 CaCl 2, 1 MgCl 2, 10 glucose, 10 HEPES at pH 7.4. Web2024-03-02 Application filed by IonOptix LLC filed Critical IonOptix LLC ... features of apparatus 10 are generally conventional and are resident in the C-Pace EP culture pacer available from IonOptix LLC of Milton, Mass. The electrical stimulation only output banks generally have the following properties: Frequency: 0.010-99 Hz;
(PDF) The cAMP binding protein Epac modulates Ca2+sparks by a …
WebFrame grabber, myopacer, acquisition, and analysis software were obtained from IonOptix (Milton, MA). After an initial 1 min basal contractility period, compounds were perfused for 5 minutes before a 2 min washout period. Web3 jan. 2024 · www.ionoptix.com IonOptix LLC 309 Hillside Street, Milton, MA 02186, USA T: 1‐617 696‐7335 F: 1‐617 698‐3553 E: [email protected] IonOptix Ltd 1a Dartmouth … inafed tlaxcala
Preconception Exposure to Fine Particulate Matter Leads to …
Web1 dec. 2002 · Fura 2 fluorescence was measured by using a photomultiplier system (IonOptix, Milton, MA) in which background-corrected ratios of the 510-nm emission from arteries alternatively excited at 340 and 380 nm were obtained at a sampling rate of 5 Hz. Arterial diameter was simultaneously recorded by using the SoftEdge Acquisition … Web1 okt. 2024 · Fluorescence emissions were detected using a fluorescence photomultiplier tube (IonOptix, Milton, MA). Alterations in fura-2 fluorescence intensity (FFI) were quantitated from the FFI ratio at 360 nm and 380 nm. Intracellular Ca2+ clearance was assessed by fluorescence decay time [ 29 ]. 2.4. Analysis of DHE fluorescence Web6 sep. 2012 · In brief, coverslips of cardiac myocytes loaded with Fura-2AM (1 µM, 20 min, room temperature) were used to monitor intracellular calcium transients. Fura-2 fluorescence was measured using an IonOptix spectrophotometer (HyperSwitch; IonOptix, Milton, MA, USA). Fura-2 was excited by light at 340-nm and 380-nm. inch cottage grangemouth