WebDAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA.It is used extensively in fluorescence microscopy.As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently … WebApr 13, 2024 · The EpCAM expression showed a significant difference between both PC3 and PC3-9 compared to all other cell lines (p < 0.01). ... CD16-, biotin-FITC, Hoechst, Cytokeratin-APC, PSMA-PerCP/Cy5.5 and ...
Hoechst Stain - Applications
WebThe Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the additional … WebWhat is the similarity/difference between Hoechst, DAPI, IP staining? DAPI can pass through a living cell membrane and Hoechst is not cell permeable when the cell is alive. PI can only mark dead or dying cells. What do immunohistochemistry (IHC) and in situ hybridization (ISH) label? clinkery
The role of the stemness index-associated signature in the analysis …
WebMinor-groove binders ( Figure 8.1.1 ), such as DAPI and the Hoechst dyes Other nucleic acid stains, including acridine orange, 7-AAD, LDS 751 and hydroxystilbamidine Figure 8.1.1 Schematic diagram showing the … WebThe Hoechst stains may be used on live or fixed cells and are often used as a substitute for another nucleic acid stain (DAPI). The key difference between them is that the … WebHoechst dyes are often used as substitutes for another nucleic acid stain called DAPI. Key differences between Hoechst dyes and DAPI are: Hoechst dyes are less toxic than DAPI, which ensures a higher viability of stained cells. The additional ethyl group of the Hoechst dyes renders them more cell-permeable. clinker vs carvel